Attribute	Specification
Brand	BioSynLab Research Compounds
Product Name	2-Methyl-AP-237 HCl
IUPAC Name	1-[2-(4-tert-butoxyphenyl)ethyl]-2-methyl-4-phenylpiperazine hydrochloride
Synonyms	2-Me-AP-237, 2-Methyl-bucinnazine, 2-Methyl-1-butyryl-4-phenylpiperazine analog
CAS Number	Not assigned (novel research compound)
Molecular Formula	C₂₃H₃₂N₂O · HCl
Molecular Weight	388.98 g/mol (hydrochloride salt)
Salt Form	Hydrochloride (1:1 stoichiometry)
Dosage Form	Compressed Pellets (Tablets)
Unit Dose	20mg ± 0.5mg
Pellet Dimensions	4mm diameter × 2mm height
Pellet Weight	60mg total (20mg API + 40mg excipients)
Purity	≥98.0% (HPLC Verified)
Appearance	White Round Pellets
Imprint	BioSynLab “2-AP” debossing
Packaging	Blister packs (10 pellets per sheet)
Storage	2-8°C, Desiccated, Light Protected
Excipients	Microcrystalline cellulose, Pregelatinized starch, Magnesium stearate
Documentation	COA, HPLC, NMR, HRMS, MSDS, Stability Data
Shipping	Temperature-Controlled, Discrete
Origin	EU/GMP Certified Manufacturing

2-Methyl-AP-237 HCl Pellets 20mg | Novel Opioid Research Compound | BioSynLab

BioSynLab presents 2-methyl-AP-237 hydrochloride in 20mg precision-dosed pellets for advanced pharmacological research and opioid receptor investigations. As a methylated derivative of AP-237 (bucinnazine), this piperazine-based opioid analog offers unique structure-activity relationship insights for researchers studying μ-opioid receptor (MOR) pharmacology, analgesic compound development, and novel psychoactive substance (NPS) analytical characterization.

Chemical Architecture & Structural Modifications

2-Methyl-AP-237 hydrochloride represents a structural analog of the piperazine opioid AP-237, incorporating a methyl substituent at the 2-position of the piperazine ring:

Core Scaffold Features:

• Piperazine Heterocycle: Six-membered ring containing two nitrogen atoms (1,4-diazacyclohexane) serving as the pharmacophore backbone
• 2-Methyl Substitution: Additional methyl group at position 2 of the piperazine ring, creating steric modification of the binding pocket interaction
• 4-Phenyl Substitution: Aromatic ring at position 4 contributing to lipophilicity and receptor affinity
• 1-(4-tert-butoxyphenethyl) Side Chain: Ether-linked aromatic system providing μ-opioid receptor selectivity

Physicochemical Properties:

• Molecular weight: 388.98 g/mol (HCl salt)
• Calculated LogP: 4.2 (highly lipophilic)
• pKa (piperazine N): ~8.5-9.0 (protonated at physiological pH)
• Melting point: 198-205°C (decomposition, HCl salt)
• Chirality: Racemic mixture (2-methyl creates stereocenter)

Structural Relationship to AP-237:
AP-237 (bucinnazine, 1-butyryl-4-cinnamylpiperazine) is a clinically utilized analgesic in certain jurisdictions. 2-Methyl-AP-237 represents a designer modification with potentially altered pharmacokinetics and receptor binding characteristics.

Pellet Formulation & Pharmaceutical Engineering

Our 20mg 2-methyl-AP-237 HCl pellets utilize direct compression technology:

Formulation Composition:

• Active Pharmaceutical Ingredient: 2-Methyl-AP-237 HCl – 20.0mg (33.3% w/w)
• Diluent: Microcrystalline cellulose (Avicel PH-102) – 30.0mg (50.0% w/w)
• Binder: Pregelatinized starch (Starch 1500) – 8.0mg (13.3% w/w)
• Lubricant: Magnesium stearate – 2.0mg (3.3% w/w)

Physical Specifications:

• Hardness: 4-6 kp (optimal for handling)
• Friability: <0.5% (minimal dust generation)
• Disintegration: 25-40 minutes (simulated gastric fluid)
• Dissolution: >80% API release within 45 minutes
• Weight uniformity: RSD <5% (n=20)

Pellet Advantages:

• Precise unit dosing eliminates weighing variability
• Improved stability vs. powder (reduced surface area)
• Enhanced handling characteristics for laboratory manipulation
• Compatible with dissolution studies and pharmaceutical research

Blister Packaging & Stability Preservation

2-Methyl-AP-237 HCl pellets are sealed in pharmaceutical-grade aluminum blisters:

Barrier Properties:

• Cold-form aluminum (CFF) construction
• Water vapor transmission: <0.01 g/m²/day
• Oxygen exclusion: <0.1 cc/m²/day
• Light blocking: >99% (UV-Vis spectrum)

Secondary Packaging:

• Tamper-evident cartons with holographic seals
• Indicating desiccant (non-contact compartment)
• Batch-coded labeling with QR-linked COA
• Temperature indicators

Stability Profile:

• Long-term: 24 months at 2-8°C (refrigerated)
• Accelerated: 6 months at 40°C/75% RH
• In-use: 30 days at room temperature once opened

Analytical Characterization & Novel Compound Verification

As a novel research chemical, BioSynLab 2-methyl-AP-237 undergoes extensive characterization:

High-Resolution Mass Spectrometry (HRMS):

• Expected [M+H]⁺: m/z 353.2587 (C₂₃H₃₃N₂O⁺, freebase)
• Mass accuracy: <5 ppm from theoretical
• Fragmentation pattern: Characteristic piperazine ring fragments

Nuclear Magnetic Resonance (NMR):

• ¹H NMR (600MHz, DMSO-d₆):
  • Aromatic protons: δ 6.8-7.4 (m, 9H)
  • Piperazine CH₂: δ 2.5-3.2 (m, 8H)
  • 2-Methyl CH₃: δ 1.15 (d, 3H, J=6.5Hz)
  • tert-butyl CH₃: δ 1.28 (s, 9H)
  • Side chain CH₂: δ 2.6-2.9 (m, 4H)
• ¹³C NMR (150MHz): 23 distinct carbon signals
• 2D NMR (COSY, HSQC, HMBC): Complete structural assignment

Chromatographic Analysis:

• HPLC-UV (254nm): ≥98.0% purity
• Related substances: <2.0% total impurities
• Residual solvents: Meets ICH Q3C
• Chiral HPLC: Enantiomeric ratio (racemic mixture)

Spectroscopic Confirmation:

• FTIR: N-H stretch (3400 cm⁻¹), aromatic C-H (3050 cm⁻¹), C-O-C ether (1080 cm⁻¹)
• UV-Vis: λmax 254nm (aromatic chromophores)
• XRPD: Crystalline form identification

Research Applications & Scientific Rationale

2-Methyl-AP-237 HCl serves as a valuable research tool for:

Opioid Pharmacology:

• μ-opioid receptor (MOR) binding affinity determination
• Structure-activity relationships (2-methyl effect on potency)
• Receptor selectivity (MOR vs. DOR vs. KOR)
• Functional assays (G-protein coupling, β-arrestin recruitment)
• Comparison with AP-237 (parent compound)

Analgesic Research:

• Thermal nociception assays (rodent tail-flick, hot plate)
• Mechanical allodynia models (neuropathic pain)
• Inflammatory pain models (formalin test)
• Tolerance development studies

Pharmacokinetic Investigations:

• Metabolic stability (hepatocyte incubations)
• CYP450 metabolism (substrate identification)
• Plasma protein binding (equilibrium dialysis)
• Blood-brain barrier penetration (LogP correlation)

Forensic & Analytical Chemistry:

• NPS detection method development (LC-MS/MS, GC-MS)
• Retention time database construction
• Isomeric differentiation from AP-237 and positional isomers
• Stability in biological matrices

Toxicological Research:

• Respiratory depression modeling (in-vitro, in-vivo)
• Structure-toxicity relationships (opioid analog series)
• Drug-drug interaction potential (CYP inhibition)

Global Distribution & Discrete Procurement

BioSynLab provides secure distribution for opioid research compounds:

Packaging Specifications:

• Primary: Amber Type I borosilicate glass
• Closure: PTFE-faced silicone septa
• Secondary: Argon-flushed aluminized barrier pouch
• Tertiary: Discrete unmarked shipping containers

Shipping Options:

• Standard: 2-8°C with gel packs, 5-7 days
• Express: Temperature-monitored, 2-3 days
• Cryogenic: -20°C for bulk orders
• Discrete: No chemical nomenclature, generic return addresses

Service Coverage:

United Kingdom: London, Manchester, Birmingham, Leeds, Glasgow, Liverpool, Sheffield, Bristol, Edinburgh, Cardiff, Belfast, Nottingham, Leicester.

European Union: Berlin, Hamburg, Munich, Cologne, Frankfurt, Amsterdam, Rotterdam, Paris, Lyon, Barcelona, Madrid, Rome, Milan, Vienna, Brussels, Prague, Warsaw.

North America: New York, Los Angeles, Chicago, Houston, Philadelphia, San Antonio, San Diego, Dallas, San Jose, Austin, Toronto, Vancouver.